Determining Genetic Variability and Taxonomy of Hibiscus rosa-sinensis through rbcL Molecular Marker

Medicinal plants have been used in traditional medicine for a long time. These contain phytochemicals that variety of medicinal properties. However, accurate identification and authentication plant species ensured their safety efficacy. DNA barcoding using molecular markers has proven to be useful method identification. The rbcL marker was detailed characterization, amplification, phylogenetic studies Hibiscus rosa-sinensis. Objective: To evaluate the therapeutic properties potential applications Methods: Samples H. rosa-sinensis were collected, isolated by Doyle method. presence confirmed gel electrophoresis, specific primers PCR amplification. results sequenced next-generation sequencing techniques. After that, neighbor-joining technique analysis obtain pairwise nucleotide distances. Gel electrophoresis samples, amplification generates successful results. Results: obtained sequence 99.7% identical previously reported gene from Based on research, discovered as closely related species. Conclusions: found viable analysis. this study demonstrated importance herbal medicine. proved reliable quality control technology business.